|
KMID : 0613820110210111607
|
|
Journal of Life Science
2011 Volume.21 No. 11 p.1607 ~ p.1611
|
|
|
Expression of Bombyx mori Transferrin Gene in Response to Oxidative Stress or Microbes
|
|
Yun Eun-Young
Kwon O-Yu
Hwang Jae-Sam
Ahn Mi-Young
Goo Tae-Won
|
|
|
Abstract
|
|
|
|
To analyze the role of Bombyx mori transferrin (BmTf) in response to microbes or oxidative stress, we investigated the level of BmTf transcripts in B. mori treated with various microbes and oxidative stress inducers. BmTf mRNA was mainly expressed in the epidermis and fat in the bodies of B. mori injected with Escherichia coli, and up regulated in response to microbes such as bacteria, fungi, or viruses, but was hardly altered in response to oxidative stress inducers such as H₂O₂, Cu, or FeCI₃. We also confirmed that BmTf mRNA expression was increased in Bm5 cells treated with ERK, PLC, PKA, PI3K, MAPK, or JNK inhibitors, respectively. To identify the major inducer of BmTf expression, we analyzed the amount of serum iron in the hemolymph of B. mori after injection or feeding with E. coli or FeCI₃. The results showed that the amount of serum iron was not changed by injection and feeding with E. coli, although BmTf mRNA was increased by injection with E. coli. On the contrary, injection and feeding with FeCI₃ significantly increased the amount of serum iron, although they did not alter the BmTf mRNA level. On the basis of these results, we assume that up-regulation of BmTf in B. mori is closely related to the defense of microorganism, and BmTf may be expressed at the basal constitutive level when it plays a role in iron metabolism by maintaining iron homeostasis and in the insect defense mechanism against oxidative stress.
|
|
|
KEYWORD
|
|
|
Bombyx mori, transferrin, iron metabolism, innate immunity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|